Subject(s)
Adjuvants, Immunologic/pharmacology , Animals , Chelating Agents/pharmacology , Disulfiram/pharmacology , Ditiocarb/pharmacology , Drug Resistance , Glutathione/metabolism , Humans , Leishmania donovani/metabolism , Leishmania major/metabolism , Leishmania tropica/metabolism , Leishmaniasis/drug therapy , Nitric Oxide/metabolism , Oxidative Stress , ATP Binding Cassette Transporter, Subfamily B/metabolismABSTRACT
Activated macrophages simultaneously synthesize nitric oxide and superoxide anion which can react with each other producing peroxynitrite. Consequently, it has been difficult to assess the precise contribution of each of the formed reactive oxygen- and nitrogenderived species to the microbicidal activities of macrophages, particularly in vivo. To explore this problem, we are examining the formation and potential roles of nitrogen-derived intermediates in Leishmania amazonensis murine infection. Thus far, our results have demonstrated that peroxynitrite is a potent leishmanicidal agent in vitro and that both nitric oxide and peroxynitrite are formed during infection of susceptible BALB/c mouse strain. Nitric oxide was detected as the nitrosyl-hemoglobin complex by electron paramagnetic resonance analysis of blood drawn from mice at different times of infection, and it was shown to increase with the evolution of the disease. These results will be discussed in the context of the dual physiological role of nitric oxide either as a signaling molecule or as a deleterious agent.